v.) application of new substances to stain or highlight dysplastic or neoplastic lesions. However, tumor models have been preferentially developed in rodents (mice and rats), while new endoscopes, particularly prototypes, are manufactured

exclusively with large diameters (~9-12 mm) appropriate for patients. Current attempts to address this issue include use of tumor models in larger animals and the development and use of small (i.e. rodent-scale) endoscopes but these have not yet progressed significantly. Dedicated small-animal endoscopes, for example, are often based on fiberoptic technology rather than on high-resolution video, the technology routinely used in clinical endoscopy (6),(7). Inhibitors,research,lifescience,medical The current Inhibitors,research,lifescience,medical study has therefore been undertaken to combine both considerations, small-animal tumor models and normal size clinical-scale endoscopes, in a single experimental setting and to thereby establish a practical method for evaluating new endoscopic techniques in small-animal models of colon carcinogenesis. The main aims of the study were Inhibitors,research,lifescience,medical to determine (i) the feasibility of the http://www.selleckchem.com/products/MLN-2238.html proposed method, and

(ii) the duration of preservation of a vital colon mucosa with intact blood supply under the study conditions described. Methods Animal tumor model The rhabdomyosarcoma cell line R1H, originally derived from the jaw musculature of a WAG/Rij rat, was provided by Dr. Annette Raabe, Department of Radiotherapy and Radio-Oncology, Inhibitors,research,lifescience,medical University Hospital of Hamburg-Eppendorf, Germany. Male WAG/Rij rats

were purchased from Charles River WIGA (Deutschland) GmbH (Sulzfeld, Germany). All experiments were kinase inhibitor Cisplatin performed according to German law at the Preclinics – Gesellschaft für präklinische Forschung mbH (Potsdam, Germany). For tumor formation, 50 µl of cell suspension of 2 x 10(7) R1H cells were injected at 4 sites into the cecal wall of 12-week-old male WAG/Rij rats (n = 6) following laparotomy under general anesthesia with isoflurane. Another male WAG/Rij rat did not receive tumor cell injections. Intraoperative endoscopy At 23 days after the Inhibitors,research,lifescience,medical cell injection, the animals underwent a second laparotomy under inhalation anesthesia with isoflurane. The cecum was then opened longitudinally. The cut was made at the side contralateral to the insertion of the mesenteric blood vessel, in order to preserve an unhindered Drug_discovery blood supply during the entire procedure. With the now exposed luminal surface upwards, the cecum was positioned on a specially designed height-adjustable table above the animal’s chest; the table also had a measuring line alongside the exposed bowel. The endoscope was fixed in an upright clamp with a screw mechanism to allow adjustment of the distance between the endoscope tip and the bowel (Fig 1). In order to simulate the dark conditions of in vivo endoscopy, an opaque curtain was fixed around the circumference of the distal portion of the endoscope.