Immersion for 30 minutes, caused by enzalutamide 915087-33-1 depolarization 96mm KCl obtained Ht dependent Ngigen voltage having reached a maximum base area of the contraction Che tension.6 0.5 g obtained Hte blood to claim 1 g and 2 g not for a further increase increase of KCl contraction. When the base voltage has been to 3 g KCl contraction reduced.6 ht Based on these findings obtained, W We hlten 0.5 g as a control voltage base which produces a maximal contraction. We have also g of 2 as the maximum voltage that produces a maximal contraction without causing too much stretching of the vein wall and Gewebesch Apology. We beautiful tzten curves Sen pressure corresponding to the basal tension of the IVC-segments using the formula PF / A, where P is the pressure in grams force/cm2, F force, A is the liquid Surface, and, starting from a middle diameter IVC are shown as 1.5 mm histologically. The pressure at 0.5 g tension generated, 28.4 g force/cm2 or 20.8 mm Hg and the pressure to 2 g voltage is generated, 113.2 grams or force/cm2 83.4mmHg. These pressures are consistent with the curves Sen pressures in the lower end of man observed that vary between 10 and 100 mm Hg, dependent Can ngig of the position and muscle contraction. Although the shops tzten pressure of 0.5 g and 2 g tension generated seemed Similar to the lower leg of the curves Sen pressure in humans, it is important to note that this was performed on the rat IVC, and, therefore, any extrapolation the results in the veins of the varicose veins from rats to humans should be interpreted with extreme caution. Vein segments were first Highest under 0.5 g tension stretched for 1 hour. To control the properties of the contraction To be determined on the IVC segments were stimulated twice with 96 mm KCl, then with PHE. Each contraction contr The IVC was followed by 3 minutes 10 w Deleted cancer. The Badel Solution was then changed in the middle of the tissue culture GE.
The CBF was then subjected by train or 0.5 g per 18 hours, the high voltage 2 g for 18 hours or 2 g of the voltage for 18 hours, most of the modulator HIF U0126, 17 DMAG, echinomycin or DMOG. IVC segments were washed in Krebs three washes with 10 minutes, and a second contraction 96mm KCl and PHE has been generated. The fold CHANGE OF contraction was calculated by dividing the contraction and after PHE L Calculated prolonged voltage for 18 hours by the contraction of control corresponding to 0.5 g tension for 1 hour KClinduced. We have already shown that an L Prolonged increase in rat IVC wall stress eingeschr for 24 hours Nkter contraction and increased Hte expression of MMP 2 and MMP 9.6 was associated in this study, we have a slightly shorter period of 18 hours stretching to m Possible negative feedback mechanisms that HIF.20 previous studies on the regulation of hypoxia HIF used k takes avoid nnten, down-regulate about 16 hours to negative feedback mechanisms, the results complicate . avoid 21 Examples of the negative feedback mechanisms that are activated by hypoxia can k Include the production of antisense RNA production of HIF 1.22 CBP/p300 interacting transactivator with ED-rich tail 2, which can bind to and block the p300 transcriptional activity for setting t of HIF, 20.23, and induction of transcription PHD, which negatively regulates HIF expression.20 real-time reverse reaction cha no transcription-polymerase. RNA was isolated from IVC using a fiber RNeasy.