The vessels’ basement membrane is positive for PAS staining (pink) (original magnification: ×400). Characteristics and follow up of patients Among the 203 patients, there were 154 men (75.86%) and 49 women (24.14%). The mean age at diagnosis was 66 years, ranging from 32 to 77 years. 166 (81.77%) cases reported history of tobacco use, and 37 (18.23%) A-1155463 cases without. 91 (44.83%) cases indicated history of alcohol consumption

and 112 (55.17%) cases without. Patients with tumors located at super glottic were 93 (45.81%) cases, at glottic were 93 (45.81%) cases, and at subglottic were 17 (8.37%) cases. Patients in pTNM stage I, II, III and IV were 25 (12.32%), 60 (29.56%), 62 (30.54%) and 56 (27.59%), respectively. Patients in different T classification T1, T2, T3 and T4 were 27 (13.30%), 93(45.81%), Epigenetics inhibitor 44(21.67%) and 39(19.21%), respectively.151(74.38%) patients showed lymph node metastasis at diagnosis, and 19 (9.36%) patients appeared to show distant metastasis postoperative. In addition, histological grade 1 was in 30 (14.78%), grade 2 was in 149 (73.40%) and grade 3 was in 24 (11.82%) cases. The mean follow-up time was 80 months (range 2-219 months). 121 patients (59.61%) were alive when the follow up ended. Eighty-two patients (40.39%) died as a result of their malignancy. The median

DFS was 56 months. Local recurrence and local lymph node metastasis was observed in 157 patients (77.34%). The mean period from initial surgery to the first local recurrence or metastasis was 63.71 months (range 1-213 months). Nineteen (9.36%) patients developed distant metastasis. The metastatic sites included lung

see more (n = 9), bone (n = 4), liver (n = 3), mediastinum (n = 2), and multiple concomitant metastasis (n = 1, including thoracic this website vertebrae, spinal cord and tibia). Clinical significance of VM in LSCC patients compared with EDV Clinical significance of VM and EDV are listed in Table 1. The positive rate of VM was significantly higher in progressive stage (III and IV) than primary stage (I and II) (27.97% vs. 12.94%) (p = 0.010) clinically, and it was significantly greater in patients with local lymph node metastases than those without local lymph node metastasis (36.53% vs. 16.56%) (p = 0.003). In addition, the positive rate of VM became higher with the raise of histopathological grade: grade 1(6.67%), grade 2 (20.13%), grade 3 (50.00%) (p < 0.0001). And the incidence of VM did not differ with respect to the patients' gender, age, tumor size, T stage, tumor location, recurrence or distant metastasis (all P > 0.05). Table 1 Comparing clinicalpathologic significance of VM and EDV factor   VM     MVD       + – χ 2 P ( ± S) F/t* P Gender     0.881 0.380   1.228* 0.269    M 34 118     17.8739 ± 6.82709        F 10 42     16.6340 ± 6.08995     Age     0.370 0.712   0.108* 0.742    ≥60 22 85     17.4393 ± 6.92216        <60 22 74     17.7514 ± 6.