The MS spectrum showed a quasi molecular ion at m/z 539 as well as fragments have been dependable together with the following fragmentation pattern: the ion at m/z 377 arose in the reduction of glucose, the ion at m/z 307 was characteristic with the loss of the C4H6O fragment along with the fragment at m/z 275 may perhaps derive through the reduction of CH3OH through the elenolic fragment from the molecule. Peak seven exhibited the pseudo molecular ion at m/z 377 in MS and characteristic ions at m/z 197 and m/z 153 in its MS2 spectrum, corresponding for the oleuropein Taxol aglycone or its isomer. By retrieving of literature data, peak seven was recognized as oleuropein aglycone. Among 51 analytes, one can find 6 phenolic acids and three diterpenoids originated from Radix Salvia Miltiorrhiza. Phenolic acids could be categorized into monomer and polymer. Polymers can be composed of 1 or a lot of various monomers including danshensu, caffeic acid or other folks. Within the MS2 spectra of a few monomer requirements, together with little molecules which include CO2, CO and H2O have been generated within the fragmentation pathways, which indicated the presence of carboxyl, carbonyl or hydroxyl groups. Danshensu showed a ion at m/z 197, and manufactured m/z 179 and m/z 135 .
Comparable to danshensu, the two from the ions at m/z 137 of protocatechuic aldehyde and m/z selleck chemicals 153 of protocatechuic acid created exactly the same ion at m/z 109 corresponding for the reduction of CO and CO2, respectively.
As to three polymers, which contained an ester bond or ester bonds, the predominant fragmentation of their ions was the cleavage from the ester bond to shed danshensu and caffeic acid . For example, peak ten exhibited a quasi molecular ion of m/z 717. Its MS2 spectra gave rise to distinguished ion at m/z 519 corresponding towards the loss of the molecule of danshensu. Other two fragment ions, ion at m/z 321 and ion at m/z 339 corresponding to the loss with the second danshensu and the first of all caffeic acid. These information are dependable with those from the literature. So, peak 10 was tentatively identified as salvianolic acid B. Similarly, peaks 9, 14 have been recognized as rosmarinic acid and salvianolic acid A separately. Rhizoma Coptids alkaloids, which have been just about the most abundant constituents during the alcohol additional of FTZ, exhibited a exceptional fragmentation pathway inside the constructive ion mode. It is well recognized that loss the neutral species such as CO, CH3, CH4 and CH2O have been observed in the MS2 spectra of Rhizoma Coptids alkaloids. Peak 23 showed a molecular ion at m/z 336 ? in MS spectra, and exhibited some ions at m/z 320 ?, 306 ?, 292 ?, 278 ?, 262 ? and 234 ? in MS2 spectra, displaying the neutral loss of CO, CH3, CH4 and CH2O in the fragmentation pathway. These information are normal for your Rhizoma Coptids alkaloids inside the present examine and constant with individuals within the literature. Consequently, the compound was identified as berberine.