Tests that are simple, reliable, reproducible, sensitive
and cost effective will become necessary with advancing instrumentation. We have described a CE-based method for differentiating Cryptosporidium species from within and between host groups. Genetic variation for other ABT-263 mouse parasitic species has been investigated using SSCP (Gasser & Chilton, 2001; Hutson et al., 2004; Mahnaz et al., 2006; Lin et al., 2007), suggesting that CE would also be useful for other parasites. We are currently assessing CE-SSCP for use with different Cryptosporidium loci and as a tool for assessing the biodiversity of this genus. Applications of this rapid method to detection, population genetics and identification will increase our understanding of the evolution and diversity of this important parasitic group. Funding for this research was provided through the Macquarie University Research Fellow Scheme and an Australian Research Council Linkage grant in collaboration with NSW Health. “
“Pregnant mothers are susceptible to bacterial infections,
which may compromise the health of mothers and offspring. Enterococcus faecalis is a ubiquitous species found in food, restaurants, and hospitals where pregnant woman frequently become exposed to this bacterium. However, the survival, distribution, translocation, and corresponding influence of E. faecalis have not been investigated during the pregnancy period, when the mother and fetus are susceptible to bacterial learn more infection. In this study, a fluorescing E. faecalis strain was used to track the fate of the bacterium in pregnant mice. Orally administered E. faecalis were found to survive and disseminate to all regions of the intestinal
tract. It also altered the bacterial community structure by significantly decreasing Decitabine cell line the diversity of Lactobacillus species, impairing the normal structure and function of the intestinal barrier, which may contribute to the bacterial translocation into the blood, spleen, placenta, and fetus. This may affect fetal and placental growth and development. “
“Predation rates were measured for two Acanthamoeba castellanii strains feeding on metal-tolerant and metal-sensitive strains of Pseudomonas putida and compared with cellular thermodynamic data. Predation rates by A. castellanii strain ATCC 30010 correlated with cell volume of the prey. To explore whether this observation could be environmentally relevant, pseudomonad species were isolated from a pristine and a metal-contaminated river and were paired based on phylogenetic and physiological relatedness. Then, cellular thermodynamics and predation rates were measured on the most similar pseudomonad pair. Under cadmium stress, the strain from contaminated river sediments, Pseudomonas sp. CF150, exited metabolic dormancy faster than its pair from pristine sediments, Pseudomonas sp. N9, but consumed available resources less efficiently (more energy was lost as heat).