Quantitation was done using multiple reaction monitoring in the positive ionization mode. The linearity of the method was established in the concentration range 0.05-80 ng/mL. The mean extraction recovery was greater than 96% across QC levels, while intra- and inter batch accuracy and precision (% CV) values ranged from 97.4 to 101.9% and from 1.20 to 3.72% respectively. The relative matrix effect in eight different lots
of plasma samples, expressed as % CV for the calculated slopes of calibration curves was 1.08%. The stability of aripiprazole was studied under different storage conditions. The validated method was used to support a bioequivalence study of 10 mg aripiprazole formulation in 36 healthy Indian subjects. (C) 2013 Elsevier B.V. All rights reserved.”
“Background: Although echocardiography is used as a first line imaging modality, its accuracy to detect acute and chronic
Repotrectinib chemical structure myocardial infarction (MI) in relation to infarct characteristics as assessed with late gadolinium enhancement cardiovascular magnetic resonance (LGE-CMR) is not well described.\n\nMethods: One-hundred-forty-one echocardiograms performed in 88 first acute ST-elevation MI (STEMI) patients, 2 (IQR1-4) days (n = 61) and 102 (IQR92-112) days post-MI (n = 80), were pooled with echocardiograms of 36 healthy controls. 61 acute and 80 chronic echocardiograms were available for analysis (53 patients had both acute and chronic echocardiograms). Two experienced echocardiographers, blinded to clinical and CMR data, randomly evaluated all 177 echocardiograms for segmental wall motion abnormalities SAR302503 (SWMA). This was compared with LGE-CMR determined infarct characteristics, performed 104 +/- 11 days post-MI. Enhancement on LGE-CMR matched the infarct-related artery territory in all patients (LAD 31%, LCx 12% and RCA 57%).\n\nResults: The sensitivity of echocardiography to detect acute MI was 78.7% and 61.3% for chronic MI; specificity was 80.6%. Undetected MI were smaller, less transmural, and less
extensive (6% [IQR3-12] vs. 15% [IQR9-24], 50 +/- 14% vs. 61 +/- 15%, 7 +/- 3 vs. 9 +/- 3 segments, p < 0.001 for all) and associated Etomoxir purchase with higher left ventricular ejection fraction (LVEF) and non-anterior location as compared to detected MI (58 +/- 5% vs. 46 +/- 7%, p < 0.001 and 82% vs. 63%, p = 0.03). After multivariate analysis, LVEF and infarct size were the strongest independent predictors of detecting chronic MI (OR 0.78 [95% CI 0.68-0.88], p < 0.001 and OR 1.22 [95% CI0.99-1.51], p = 0.06, respectively). Increasing infarct transmurality was associated with increasing SWMA (p < 0.001).\n\nConclusions: In patients presenting with STEMI, and thus a high likelihood of SWMA, the sensitivity of echocardiography to detect SWMA was higher in the acute than the chronic phase.