Protein content in untreated and polymyxin B-treated culture frac

Protein content in untreated and polymyxin B-treated culture SIS3 price fractions are similar. Equivalent volumes of sub-cellular fractions from untreated (A) and 0.75 μg/mL polymyxin B-treated (2 h, 37°C) (B) log-phase cultures of MK496 were separated by SDS-PAGE and stained using SYPRO Ruby Red. Whole cell (WC), cytoplasm

(C), inner membrane (IM), periplasm (PP), outer membrane (OM), and OMV fractions were isolated and purified using previously described methods [53]. The protein content and protein ratios in each fraction are very similar for both conditions. (n = 3). (JPEG 173 KB) References 1. McDermott PF, Walker RD, White DG: Antimicrobials: modes of action and mechanisms of resistance. Int J Toxicol 2003,22(2):135–143.PubMedCrossRef 2. Kulp A, Kuehn MJ: Proteasome inhibitor Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Annu Rev Microbiol 2010, 64:163–184.PubMedCrossRef 3. Ellis TN, Kuehn MJ: Virulence and immunomodulatory

Selleckchem CBL-0137 roles of bacterial outer membrane vesicles. Microbiol Mol Biol Rev 2010,74(1):81–94.PubMedCrossRef 4. Kobayashi H, Uematsu K, Hirayama H, Horikoshi K: Novel toluene elimination system in a toluene-tolerant microorganism. J Bacteriol 2000,182(22):6451–6455.PubMedCrossRef 5. Yonezawa H, Osaki T, Kurata S, Fukuda M, Kawakami H, Ochiai K, Hanawa T, Kamiya S: Outer membrane vesicles of Helicobacter pylori TK1402 are involved in biofilm formation. BMC Microbiol 2009, 9:197.PubMedCrossRef 6. Schooling SR, Beveridge TJ: Membrane vesicles: an overlooked component of the matrices of biofilms. J Bacteriol 2006,188(16):5945–5957.PubMedCrossRef 7. Grenier D, Belanger M: Protective effect of Porphyromonas gingivalis outer membrane vesicles against bactericidal activity of human serum. Infect Immun 1991,59(9):3004–3008.PubMed 8. Grenier D, Bertrand J, Mayrand D: Porphyromonas gingivalis outer membrane vesicles promote bacterial resistance to chlorhexidine. Oral Microbiol Pyruvate dehydrogenase lipoamide kinase isozyme 1 Immunol 1995,10(5):319–320.PubMedCrossRef 9. McBroom AJ, Kuehn MJ: Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response. Mol

Microbiol 2007,63(2):545–558.PubMedCrossRef 10. De Smet K, Contreras R: Human antimicrobial peptides: defensins, cathelicidins and histatins. Biotechnol Lett 2005,27(18):1337–1347.PubMedCrossRef 11. Svetoch EA, Stern NJ, Eruslanov BV, Kovalev YN, Volodina LI, Perelygin VV, Mitsevich EV, Mitsevich IP, Pokhilenko VD, Borzenkov VN, et al.: Isolation of Bacillus circulans and Paenibacillus polymyxa strains inhibitory to Campylobacter jejuni and characterization of associated bacteriocins. J Food Prot 2005,68(1):11–17.PubMed 12. Harris F, Dennison SR, Phoenix DA: Anionic antimicrobial peptides from eukaryotic organisms. Curr Protein Pept Sci 2009,10(6):585–606.PubMedCrossRef 13. Chopra I: The magainins: antimicrobial peptides with potential for topical application. J Antimicrob Chemother 1993,32(3):351–353.PubMedCrossRef 14.

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