Probes that did not produce a significant match with any other eukaryotic mRNAgene were Calcitriol clinical trial excluded from gene lists used for functional analysis. Throughout this manuscript official human gene symbols were used in the text and in all figures and tables. Response Inhibitors,Modulators,Libraries genes detected in inter animal comparisons were assigned to a list of genes based on their similarity in response to cytokines IL8 at 2 hours and IL1B at 4 hours. Because of the important role of IL8 and IL1B in inflammatory processes, these lists were named after these cytokines. Lists of gene symbols were uploaded separately in web based bioinformatics programs. The Database for Annotation, Visualization and Inte grated Discovery website and the Set Distiller module of GeneDecks were used to assign genes to a specific pathway.

Because far more human genes are annotated, and more information in databases is available for humans than for pigs, the human background was used for this functional analysis. In DAVID pathways with a p value of 0. 2 were retrieved. In Genedecks path ways were retrieved called signifi cant with a p value 0. 05 using the Set Distiller algo rithm. KEGG pathways Inhibitors,Modulators,Libraries retrieved from DAVID were only listed when not called significant by Genedecks, or in case more genes were listed than in Genedecks. In case a pathway was called significant in one group of genes, genes regulated on the same time point in the opposite list, and also part of this pathway, were also retrieved from DAVID and GeneDecks.

Inhibitors,Modulators,Libraries From DAVID Functional Annotation charts transcrip tion factors or genes involved Inhibitors,Modulators,Libraries in regulation of transcription were identified by gene ontology analysis and uploaded as sub list in GNCPro to establish relations be tween these genes. Non interacting genes were omitted Inhibitors,Modulators,Libraries from the displayed network. Functional association between proteins encoded by differential expressed genes, ligands, and enzyme substratesproducts linked to these proteins, were established using the protein chemical inter action web tool STITCH2. Relevant chemicals were added to gene lists and uploaded to in STITCH2 to establish associations. Associations with a confidence score of 0. 4 were selected from output files and displayed. In Additional file 1 Table S1 the type and confidence level of each association is listed http://www.selleckchem.com/products/CP-690550.html in a separate sheet STITCH interactions. Chemical data mining Based on bioinformatics analysis a set of reg ulated genes was selected from 8, 4 and 2 hours micro array comparisons. Each individual geneprotein was loaded into STITCH2 to find associations with chemical compounds scoring a high confidence level.