And plenty of sperm in the lumen, respectively. In the development of the ovary, steps one through five as Eierst tance using germ cells into mature oocytes are described: Oogonia and primary Ren oocytes, cortical alveoli, early vitellogenic oocytes, sp t vitellogenic oocytes and mature oocytes / eggs are. Morphology of the reproductive organs was defined as an attachment double or single rooms, or not assessable. In some cases F To determine the nature of the reproductive organs has not m Been possible through the plane of the section or distortion of the behavior of the gonads. 2.6. The quantification of the secondary Ren sexual characteristics, the number and importance of marital tubercles on the m Nnliche and female fish were recorded in all surviving fish F0 and F1, as they have shown there it on hormones stero Of confinement Lich anti-androgens. The Z Select and classify the marital tubercles on the work of Smith, which is based in the classification with respect to the ongoing importance of the tubers. 2.7. Analytical concentrations in the water chemistry of bicalutamide was measured in all ponds at baseline and subsequently every week alternately in tanks or containers Lter for Minderj Hrige. L Surplus stock solutions were also analyzed per week. w uncircumcised samples were analyzed by high performance liquid chromatography. In summary, the separation on a 50 mm 1.0 mm  XBridge 3.5 m C18-S column at a rate of 150 l was min -1 and an S performed ulentemperatur of eluents C 50: 0.10% ammonia in water and acetonitrile, in using a gradient elution follows: 0 min 10% B, 4 min 100% B, 5 min at 100% B, 5, 01 min 10% B and 6.5 min at 10% B. A mass p38 MAPK Signaling Pathway spectrometer TSQ Quantum Access operated in negative ionization electrospray mode heated, with a spray voltage of 4.0 kV, capillary voltage of 35 V, capillary temperature of 275 ° C and the sheath gas and auxiliary 30 and 5 arbitrary units. Deprotonated molecular ions
of bicalutamide was m / z 428.85, controlled The weight Hlten reaction conditions by monitoring product ion m / z 254.86 and m / z 184.90. Further details of the method of analysis can be obtained from the corresponding author. 2.8. The analysis of the statistical analysis of F0 means the production of eggs per pair, the number of eggs per spawning pair and size S account of the fish, which w Died during the study. The data were assessed for normality and homogeneity t of the variances between the treatments. In any case, the assumptions of normality T homogeneity and t were met, was followed by an ANOVA and Dunnett’s test of art. When the assumptions were not met, the data were fitted using a parametric test, followed, for example, Kruskal Wallis, through a series of tests Mann-Whitney with Bonferroni adjustment of the significance level. F0 and F1 data for wet weight, standard L Length and number of nuptial flight tubercles were for normality Homogeneity and t t the variances evaluated. Where assumptions of normality T homogeneity and t were met, the variance was followed by Dunnett’s test to compare the treatment with controlled means The relevant. If the assumptions were not met, the data were analyzed using Kruskal-Wallis analysis. Nuptial tubercles prominent and G-Residents F1 eggs were analyzed for statistical significance by contingency table analysis using the likelihood ratio test for contingency protocol.