“
“Objective: To describe a simple technique for pediatric cochlear implant receiver-stimulators (R/S) fixation and long term results with it.
Methods: Clinical sample include thirty-two children with severe-to-profound or profound hearing loss candidate for
cochlear implantation. Low-profile devices were used (Med El Concerto (c) and Nucleus CI512 (c)). The R/S was housed into a tight subperiosteal pocket carefully dissected behind the mastoidectomy. The pocket was closed with a single periosteal to bone suture around the posterior edge of mastoidectomy. No skull drilling was required for housing the device.
Results: No intraoperative complications have been observed. 20% of operating time was saved. No migration of R/S over time was apparent at follow up.
Conclusions: The back-pocket technique seems effective in retaining the CI package
Fosbretabulin and in optimizing surgical time and safety in pediatric cochlear implantation. It seems particularly suitable with new generation low profile CI devices. (c) 2013 Elsevier Ireland Silmitasertib Ltd. All rights reserved.”
“Introduction: Development of renal biomarkers is required to improve on diagnostic accuracy, prognosis and prediction of response to therapy in renal disease. We describe a new method of obtaining from renal specimens a biologic fluid potentially enriched in secreted proteins.
Methods: A renal biopsy specimen was centrifuged, and the LGX818 price interstitial fluid (IF) obtained was evaluated by SELDI-ToF, 1D and 2D gel electrophoresis. Twelve spots were extracted from the
2D gel and characterized by MALDI-TOF-MS.
Results: The SELDI diagrams demonstrated abundant peptide peaks. One-dimensional gel electrophoresis demonstrated the presence of many bands indicating a diversity of proteins in the sample. Comparison of serum to IF demonstrated a number of bands that were not shared, suggesting that the IF is not a simple “”replica”" of plasma fluid. Employing 2D-PAGE, 418 spots were identified in the IF sample; 12 spots were selected and analyzed by mass spectrometry.
Conclusions: We have described a novel technique to obtain a biologic fluid that contains a significant quantity and diversity of proteins from renal tissue. The procedure to obtain the fluid is simple and easily applicable to standard renal biopsy procedures. This fluid has the potential to identify informative proteins that are more concentrated than in any other renal biologic fluid previously analyzed and strictly related to renal pathophysiology. Future work includes the development of a clinical protocol to identify and validate informative biomarkers that have diagnostic and prognostic value.