Mean SOFA score immediately after CPR was 11.0 �� 0.4 and 10.6 �� 0.4 24 hours later (Table (Table1).1). Average selleckbio time from ROSC to first blood sampling was three hours and 42 minutes �� 48 minutes. The second blood sample was collected 25 hours and 48 minutes �� 42 minutes after ROSC.Patients of the resuscitation and cardiological control group were comparable in baseline characteristics such as gender and age, as well as presence of a significant coronary artery disease (CAD) in medical history and proportion of subjects, which underwent coronary angiography during the blood sampling period of about 26 hours (Table (Table1).1). There were slight differences in cardiovascular risk profiles between the two groups with a higher incidence of hyperlipidemia in the control group.

Resuscitated patients showed elevated leukocyte count and C-reactive protein levels but comparable platelet count compared with controls. Patients in the CPR group presented significantly higher incidence of acute renal failure, compared with control patients (Table (Table11).All measurements were also performed in 15 healthy controls. Age at time of the study was 31.3 �� 1.9 years.MMPs and procoagulant PMPsThe mean number of MMPs in CPR patients was significantly increased immediately after ROSC compared with controls being hospitalized for a cardiac cause (mean number 2.2 �� 0.4 vs. 0.3 �� 0.06 events/��L; P < 0.01) or healthy controls (mean number 0.5 �� 0.1 events/��L; P < 0.05) and persisted in the 24 hours follow up (mean number vs. control: 2.2 �� 0.8 vs. 0.5 �� 0.2 events/��L; P < 0.

05; or vs. healthy: 0.5 �� 0.2 events/��L; P < 0.05; Figure Figure2).2). In all groups, there was no significant change in MMP count in the 24 hour follow up (CPR: P = 0.22; control: P = 0.85; healthy: P = 0.48) and there was no correlation between monocyte count and MMP count detectable (r2 = 0.01; P = 0.78).Figure 2Elevated monocyte-derived microparticles in patients after CPR. Significant elevation of monocyte-derived microparticles (MMPs) in peripheral blood of resuscitated patients (cardiopulmonary resuscitation (CPR); black bars) immediately (left) and 24 hours ...Similarly procoagulant PMP levels were elevated in resuscitated patients. Whereas the mean number (35.1 �� 16.2 events/��L) was only significantly elevated compared with healthy subjects immediately after CPR (mean number 6.

2 �� 2.9 events/��L; P < 0.05), there was only a slight but not significant increase detectable compared with the cardiological control group (mean number 22.8 �� 11.2 events/��L; P = 0.33). Twenty four hours after ROSC the mean number of procoagulant PMPs (67.9 �� 25.6 events/��L) in resuscitated patients was significantly higher Drug_discovery than in the cardiological control group (mean number 11.3 �� 1.7 events/��L; P < 0.01) and in healthy subjects (mean number 6.4 �� 2.0 events/��L; P < 0.005; Figure Figure3).3).