coucha papillomavirus 2 (McPV2). Both viruses are spread widely within the colony at the German Cancer Research Center. The animals are unique in that they spontaneously develop multiple epithelial tumors such as MnPV-induced papillomas and McPV2-induced condylomas. The humoral immune response of a cohort of 98

Mastomys was analysed and revealed a high prevalence of antibodies to L1 (MnPV: 36.7%, McPV2: 52.0%). Furthermore, the seroreactivity to both viruses was significantly increased buy Idasanutlin in animals with the respective tumors (22 papillomas, 21 condylomas) as compared to 55 tumor-free controls (MnPV: p < 0.0001; McPV2: p = 0.0022). The identified assay conditions showed a high level of sensitivity (MnPV: 75.7%; McPV2: 85.7%) and reproducibility (MnPV: R(2) = 0.83; McPV2: R(2) = 0.79), validating the GST-capture ELISA as a powerful method for monitoring papillomavirus serology in M. coucha. (C( 2009 Elsevier B.V. All rights reserved.”
“The emerging importance of criniviruses, together with their limited characterisation, necessitates the development of simple tools to enable rapid detection and monitoring in case of an outbreak. While serological tools would be ideal, criniviruses are notoriously difficult to purify and traditional methods of antibody production, requiring purified virus particles, are extremely

MEK162 mouse challenging. The development of a novel molecular strategy

for in planta viral antigen preparation to bypass particle purification and allow antibody production are described. An A. tumefaciens-mediated transient expression system, coupled with a green fluorescent protein (GFP) purification method was employed to generate CYSDV coat protein (CP) in whole plant leaves. The CYSDV CP gene was ligated into a GFP construct, transformed into A. tumefaciens and agroinfiltrated into N. benthamiana. Expression levels of the recombinant protein were increased by co-infiltration with the viral gene-silencing suppressor P19 from TBSV. The recombinant protein, purified from plant leaves was used to immunise rats for the preparation of polyclonal antisera. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.”
“Previous AICAR in vivo and new PCRs for KHV detection were compared by estimation of their sensitivity in recognizing KHV DNA in plasmids, cell culture extracted KHV DNA and total DNA obtained from field tissue samples. A modified real-time PCR (Gilad et al., 2004), combined with an internal control system (IC2, Hoffmann et al., 2006) in a duplex assay, was used as a “”gold standard”". The lowest reliably determined virus concentration between, 5 and 10 KHV DNA genomic equivalents, was found by real-time PCR (Gilad et al., 2004), nested PCR (Bergmann et al., 2006) and one-tube semi-nested PCR.