Dose-dependent-Dependent manner with IC50 values of 6.0 and 5.5 m, and the activity of t Mouse ACAT liver microsomes in a dose–Dependent manner with IC50 values of 1.5 M for two compounds. In addition, inhibited the ACAT activity t in microsomes of human Caco 2 cells Hnlichen IC50 values. Under the same conditions showed the st Tested strongest beauvericin ACATactivity Cilomilast Ariflo inhibition in microsomes from all sources. These data showed that beauveriolides m Moderately inhibit ACAT 1 and 2 Hnlicher performance. Inhibition of knockout M usen ApoE Atherosclerogenesis beauveriolide. Usen after oral administration of 2 months beauveriolide III ApoE knockout-M was the atherosclerotic L Completely mission field in the aortic arch region Constantly reduced by 55% compared to the control group.
Reduction of atherosclerotic L Emissions was also in all regions of the aorta, the auff Shown lligste difference in the proximal part of the aorta. Defects sectional hearts treated group III significantly beauveriolide smaller than the control group. No significant differences in the K Occurred Resveratrol body weight, blood Figure 4 Inhibition of ACAT activity of t In the membrane fraction of mouse macrophages and mouse liver microsomes of beauveriolides I and III. Nozzles liver of M Or mouse peritoneal macrophages in 3 ml cold buffered sucrose, containing 100 mM sucrose, 50 mMKCl, 40mMKH2PO4 30mMEDTA and were suspended in a Teflon homogenizer. The liver microsome fraction and the membrane fraction of macrophages, prepared as described in Materials and Methods were used as enzyme source.
ACAT activity T was in an assay mixture, which tested 2.5 mg ml BSA in buffer A and 20 M Ls Ure with beauveriolide I or III, and the microsomal fraction or the membrane fraction. After incubation for 5 min in 37 CE was separated by TLC and the radioactivity T was measured by a radio scanner, as described in Materials and Methods. FIG fifth III beauveriolide effect on aortic atherosclerosis in apoE Mice /. ApoE / Mice were fed with 0.15% cholesterol with or without beauveriolide III for 2 months. Remember aortic sudan IV skin lesions stained apoE / mice that re u 0.05% CM-cellulose with sodium beauveriolide III and only 0.05% sodium CM. Cross sections of the aortic root L Sion couple heart shows Oil Red OF Staining in apoE / M With beauveriolide use III treated and is embroidered.
Compare the size E the entire surface Surface of the aorta for A and B, and from injury sectional drawing C and D between control and stressed beauveriolide III treated groups. ApoE / Mice of di th With or without 0.15% beauveriolide III cholesterolsupplemented fed for 2 months. Repr bar shows the mean values and error bars SD, P 0.01, P 0.05 sentieren. Glucose, total cholesterol in plasma, plasma triglycerides and fatty Plasma free acids between the two groups. Similarly, the entire aorta and atherosclerotic heart of M Nozzles treated with LDL Rknockout beauveriolide III is also reduced by 40% and 60% respectively. Zus Tzlich treated M Usen beauveriolide had observed no side effects such as diarrhea or cytotoxicity t To adrenal tissues w During trials than many synthetic ACAT inhibitors. Discussion Several beauveriolide