AT9283 of the cleaning by pr Chromatography of EGCG operated at a flow rate

Cular recognition AT9283 behavior of the synthesized polymers was prepared from 2 ml of standard L Solutions tested to work with various concentrations of quercetin by MISPE and blank Respondent Corre. 3:1 acetonitrile: The polymers were previously conditioned with 5 ml of acetone. Quercetin held at the S Column was then column of the S Eluted with 4 ml of elu ent. Different mixtures of solvents L Were vinegar Acid, methanol, methanol 5% formic Acid, 5% methanol water formic acid As with the best performance selected Tested hlt. After each step, the eluate was collected and analyzed. The specificity t of the polymers then judged by a factor of pressure KpMIP I / KpNIP, where Kp Sb / Xf, the amount of Sb with the polymer substrate and Xf of the substrate concentration in the L Solution remaining after adsorption of bound polymer.
Thus, Prei, and I BIRB 796 used to COLUMNS MIP for more specific adsorption of quercetin to beautiful. Selectivity was tested t of the MIP to a more precise structure of quercetin com books and non-relatives. 2 ml of 25 mg L L were Investigated solution in acetonitrile, acetone, individually and as a mixture of compounds loaded onto the cartridge, elected, Selected just increments and analyzed col. The selectivity of t of S and S are I1/I2 where I1 and I2 are the factors that ING Stellfl Surface for two different substrates, the factor I1 for quercetin printed defines judged. The maximum amount that can be loaded on the vehicle MIP cartridges without breakthrough of the analyte by loading two different volumes of Standardl Solutions studied business Protected.
Increasing amounts of C and EC-L solutions, 0.04 mg of each antioxidant were loaded onto 200 mg of MIP. The data were analyzed graphically. neighboring peaks. Fig. 6A and B show the chromatograms of the cleaning by pr Chromatography of EGCG operated at a flow rate of 2 ml / min and 4 ml / min, respectively. Tea catechins and caffeine were separated into four zones and collected in four fractions. Ml min for the case of 4 /, the three-phase elution gradient program set to as follows: acetonitrile / methanol / acetic acid 90/10/1 0 20 min in the first stage, 100/0/1 in 20 67.5 minutes for the second stage and 0/100/1 67.5 min after the third step, and the time intervals to collect four fractions were also adjusted accordingly. HPLC analysis of fractions collected from the figure.
6B is shown in FIG. 7 and results of quantitative analysis are shown in Table 6. It can be seen that the elution order EGC ECG EC CA and EGCG was the F1 fraction contains Lt Haupts Chlich CA and EC, the F2 fraction contains lt Haupts Chlich EGC and ECG, and contains the F3 fraction lt Haupts be chlich EGCG. It should be noted that catechins masses were concentrated caffeine and tea in the sum of all fractions are relative to their respective Subject Excursions in Ausgangsl Held solution. When the flow to 4 ml / min increased Is ht, because he had more time to the product of the fraction F3 EGCG, purity and recovery of EGCG reached 89.43% and 89.01% gain to be. In addition, the productivity was t EGCG for only about 6 mg per injection, which are by increasing Ht increase of the chromatographic system increased. Work in this direction is currently underway. 4th Conclusions A Adso silica

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