As the latter two species could not be differentiated from each other on tRFLP analysis and since both species could not be cultured in 9
cases, their presence is further referred to as L. gasseri/L. iners. Table 3 Composition OICR-9429 solubility dmso of grade I microbiota according to culture and tRFLP in the first pregnancy trimester (n = 77) L. crispatus (only) 23.4% (18) L. jensenii (only) 3.9% (3) L. gasseri/L. iners (only) 40.3% (31) L. crispatus + L. jensenii 15.6% (12) L. crispatus + L. gasseri/L. iners 9.1% (7) L. jensenii + L. gasseri/L. iners 3.9% (3) L. crispatus + L. jensenii + L. gasseri/L. iners 2.6% (2) unidentified 1.3% (1) L. crispatus,L. jensenii, and L. gasseri/iners were present with 39, 20, and 43 women in the first trimester respectively. When Target Selective Inhibitor Library cost accounting for the entire follow-up period, L. crispatus persisted at a rate
of 92.3%, L. jensenii at a rate of 80.0% and L. gasseri/iners at a rate 69.8% (Table 4). Table 4 Overview of the prevalence of the Lactobacillus index species at three consecutive points in time during pregnancy for the 77 women with grade I microflora during the first trimester Lactobacillus species as determined through culture and tRFLP (N = 77) trimester I (n) trimester II (n) trimester III (n) all samples with an L. crispatus TRF 39 (100%) 37 (94.9%) 36 (92.3%) all samples with an L. jensenii TRF 20 (100%) 18 (90.0%) 16 (80.0%) all samples with an L. gasseri/iners TRF 43 (100%) 36 (83.7%) 30 (69.8%) We subsequently accounted for changes in the prevalence of Lactobacillus index species by accounting for the first-to-second and second-to-third trimester transitions selleck compound respectively. L. crispatus was present in 39 respectively 44 women with grade I VMF during Dimethyl sulfoxide the first respectively
second trimester. When accounting for the first-to-second and second-to-third trimester transitions respectively, L. crispatus disappeared twice (5.1%) respectively once (2.3%). So, overall, L. crispatus as a member of the normal VMF (n = 83) persisted in the vast majority of cases (96.4%) throughout the following trimester. L. jensenii in turn was present in 20 respectively 22 women with grade I VMF during the first respectively second trimester. When accounting for the first-to-second and second-to-third trimester conversions respectively, L. jensenii disappeared on two (10.0%) respectively five occasions (22.7%). So, overall, L. jensenii occurring with normal VMF (n = 42), sustained throughout a subsequent trimester at a rate of 83.3%. Hence, L. jensenii was found to be a significantly less stable microflora component as compared to L. crispatus, with the likelihood of L. jensenii disappearing equalling a McNemar odds ratio of 11.67 (95% CI 3.45 – 47.51, p < 0.001). L. gasseri and/or L. iners – designated L. gasseri/iners – were present in 43 respectively 40 women with grade I VMF during the first respectively second trimester. When accounting for the first-to-second and second-to-third trimester conversions, L.