A previous study reported that P. elgii SD17 appeared to produce depsipeptide antibiotics of the polypeptin family, and yet no data on the structure elucidation of these compounds have been reported (Kim et al., 2005). In this study, the antibiotics produced
by P. elgii SD17 were also preliminarily investigated by HPLC and MS analysis. The results showed that one fraction with antimicrobial activity had the same retention time and molecular mass as Pelgipeptin B, suggesting that P. elgii SD17 could indeed produce an antibiotic of the polypeptin family. Pelgipeptins displayed strong antifungal activity in vitro against several soil-borne fungal pathogens, with MIC values of 6.25–50 μg mL−1. All of these fungi can cause devastating losses in agricultural production Maraviroc nmr throughout the world. For instance, R. solani is a widespread soil-borne fungal pathogen, which
affects many important agricultural and horticultural Fulvestrant crops worldwide. According to statistics, 24–50% economic losses across the rice cultivation zones of the world have been caused by this pathogen (Padaria & Singh, 2009). In the preliminary evaluation of in vivo efficacy, application of the n-butanol extract of P. elgii B69 containing approximately 250 μg mL−1 Pelgipeptins effectively inhibited the development of sheath blight caused by R. solani on rice, with approximately an 82% reduction in symptoms. In addition, these antimicrobial compounds in the CFS were relatively thermally stable, and showed inhibitory activity over a wide pH range,
implying that Pelgipeptins were potentially useful for the biocontrol of some plant diseases. We thank Xin-Hang Jiang, College of Life Sciences, Zhejiang University, for providing the MS measurements. “
“Sixteen lytic bacteriophages that infect Pseudomonas tolaasii LMG 2342T were isolated from smashed sporocarps of oyster mushroom (Pleurotus ostreatus) showing necrotic symptoms. On the basis of the host range investigation of the phages, they have wide infection abilities against the genus Pseudomonas, mainly in the case of phages Bf3, Bf7, Bf10, and Bf15. Molecular investigations have revealed that they all have dsDNA genomes about 40 kbp Tryptophan synthase in size. Identical restriction patterns resulting from restriction enzyme analysis suggest that the isolates probably belong to the same phage species. However, there was a difference between these phage isolates in their infecting abilities. Phage isolate Bf7 was investigated and characterized more deeply. Morphological characterization of Bf7 by transmission electron microscopy (TEM) has shown that it has a short, noncontractile tail, an icosahedral phage head, and the size is about 60 nm in diameter, suggesting that it belongs to the Podoviridae family. Complete genome sequence analysis of the Bf7 phage isolate revealed a 40 058 bp genome, 58.