, 2002). There was an approximately 50-fold purification in the case of Vibrio fluvialis hemolysin (Han et al., 2002). An approximately 15-fold, Apoptosis Compound Library supplier 22-fold, 30-fold, 50-fold and 130-fold purification was achieved for eryngeolysin (Ngai & Ng, 2006), aegerolysin, ostreolysin (Berne et al., 2002), V. fluvialis hemolysin (Han et al., 2002) and schizolysin (this study), respectively. Among the various ions examined, only Pb2+, Fe3+, Al3+, Zn2+, Hg2+ and Cu2+ inhibited
the hemolytic activity of schizolysin. Among them, the latter two metal ions showed a strongly inhibitory effect only at a concentration of 0.625 mmol L−1. Little or no inhibitory effect was detected when the following ions were tested at 10 mM: Ca2+, Mn2+, Mg2+ and Co2+ (Table 2). Sucrose and raffinose at 20 mM concentration inhibited the hemolytic activity of hemolysin by over 70%. Other sugars tested at 20 mM had little or no effect. They included α-melibiose, α-xylose,
ribose, l+-arabinose, d-galactose, SCH772984 clinical trial sorbose, glucose, mannose and O-nitrophenyl-β-d-galactopyranoside. The hemolytic activity was reduced by about 30% by fructose and inositol, by about 40% by lactose, maltose, rhamnose and cellobiose, and by about 60% by inulin (Table 3). Hemolysis induced by schizolysin was osmotically protected by a mean hydrated diameter close to 3.6 nm by PEG 4000, but not by PEG 1500, PEG 6000, PEG 10000 or PEG 20000. The reducing agent dithiothreitol significantly inhibited the hemolytic activity (Table S2). The results were different from those of other bacterial hemolysins previously reported, such as Streptococcus suis (Jacobs et al., 1994; Gottschalk et al., 1995). It is surprising that in contrast to dithiothreitol, the reducing agents
cysteine and mercaptoethanol had hardly any effect on the hemolytic activity of schizolysin. Schizolysin was tested over the pH range 5.5–8.0. The optimal pH was 6. There was a decrement in activity when the pH was raised to 8. About 20% of the optimal activity remained at pH 8 (Fig. 2a). The protein was stable between 20 and 40 °C, but its activity underwent a precipitous decline when the temperature was O-methylated flavonoid raised to 50 °C. Only about 5% activity remained at 60 °C. At and beyond 70 °C, no activity was detectable (Fig. 2b). The hemolysin inhibited HIV-1 RT with an IC50 of 1.8 μM (Table 4) but there was no inhibitory effect on the mycelial growth of several fungal species (data not shown). The hemolysin eryngeolysin displays close resemblance to other mushroom hemolysins including P. ostreatus and A. cylindracea hemolysins (Berne et al., 2002) but less similarity to fungal and bacterial hemolysins (Han et al., 2002). The molecular masses of these hemolysins are similar. Schizolysin has an N-terminal amino acid sequence and a molecular mass distinctly different from the fungal and bacterial hemolysins referred to above.