0 (SPSS IBM, Armonk, NY, USA), except the limits of agreement analysis which was conducted using MS Excel 2010 (Microsoft, London, UK). The self-selected pacing strategy inherent with the t-6MWT produced significantly different speeds (Fig. 1A) during the protocol (f   = 12.26, p   < 0.001). This was characterised by a conservatively paced minute 1 which was significantly slower than any other time during the protocol (p   < 0.05). Speed between minutes 2 and 4 was unchanged (p   > 0.05), but increased at minute 5 (p   = 0.004). Whilst there was not a continued increase to the end of the test, the speed at minute 6 was significantly selleck chemical greater than minutes 1–4

(p   < 0.05). The V˙O2 response ( Fig. 1B) was significantly increased during the protocol (f

  = 94.03, p   < 0.001) and correlated to the speed profile (r   = 0.779, p   < 0.001). Significant increases occurred at minutes 2 (p   < 0.001), 5 (p   = 0.002), and 6 (p   = 0.030). http://www.selleckchem.com/products/XL184.html Conversely BR ( Fig. 1C) significantly declined (f   = 91.14, p   < 0.001) and was negatively correlated with both V˙O2 (r = −0.686, p < 0.001) and self-selected walk speed (r = −0.411, p < 0.001). Decreases were observed each minute (p < 0.01) except between minutes 3 and 4 (p > 0.05). The mean ± SD 6MWD and 6MWW achieved during the t-6MWT was reported at 749.53 ± 94.30 m and 49,714.21 ± 9173.0 kg/m, respectively. For the most part 6MWW was more strongly related to the demographic and resting pulmonary function variables than 6MWD (Table 2). Correlation coefficients for V˙O2 compared to 6MWD and 6MWW were also assessed. The

6MWD portrayed a moderate correlation (r   = 0.656, p   < 0.001) with minute-to-minute V˙O2 ( Fig. 2A), but a slightly stronger relationship ( Fig. 2B) was observed between 6MWW and V˙O2 (r = 0.703, p < 0.001). Moves detected by the MWK were compared to 6MWD (Fig. 3A) and 6MWW (Fig. 3B), and a significant correlation was established with 6MWD (r   = −0.847, p   < 0.001) but not with 6MWW (r   = 0.337, p   = 0.220). Mean V˙O2 showed a moderate relationship (r = −0.753, p = 0.001) to moves accrued over the t-6MWT. The MWKEE demonstrated a notable relationship to 6MWW (r = 0.938, p < 0.001) ( Fig. 3D), but this was not the case between Idoxuridine MWKEE and 6MWD (r = 0.477, p = 0.720) ( Fig. 3C). The duration of time spent at different exercise intensities (Fig. 4) were significantly different between methods of calculation for moderate (χ2 = 15.25, p < 0.001) and vigorous intensities (χ2 = 19.63, p < 0.001), but not light intensity (χ2 = 4.00, p = 0.135). The MWK was not significantly different to measured (p > 0.05), but this was not the case for the standard method, which underestimated time durations at moderate intensity (p < 0.01) and over estimated at vigorous intensity (p < 0.01). Despite a strong correlation between MWKEE and gas analysis EE (r = 0.949, p < 0.001), the total EE was significantly different between the two methods (t = −6.76, p < 0.001).