Eukaryot Cell2007,6(1):73–83.CrossRefPubMed 42. Bhattacharjee S, van Ooij C, Balu B, Adams JH, Haldar K:Maurer’s clefts of Plasmodium falciparum are secretory organelles that concentrate virulence protein reporters for delivery to the host erythrocyte. Blood2008,111(4):2418–2426.CrossRefPubMed
43. Fidock DA, Wellems TE:Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil. Proc Natl Acad Sci USA1997,94(20):10931–10936.CrossRefPubMed selleck kinase inhibitor 44. Wickham ME, Rug M, Ralph SA, Klonis N, McFadden GI, Tilley L, Cowman AF:Trafficking and assembly of the cytoadherence complex in Plasmodium falciparum -infected human erythrocytes. Embo J2001,20(20):5636–5649.CrossRefPubMed 45. Mamoun CB, Gluzman IY, Goyard S, Beverley SM, Goldberg DE:A set of independent selectable
markers for transfection of the human malaria parasite Plasmodium falciparum.Proc Natl Acad Sci USA1999,96(15):8716–8720.CrossRefPubMed 46. Kadekoppala M, Kline K, Akompong T, Haldar K:Stable expression of a new chimeric fluorescent reporter in the human malaria parasite Plasmodium falciparum.Infect Immun2000,68(4):2328–2332.CrossRefPubMed 47. Li Q, Gerena L, Xie L, Zhang J, Kyle D, Milhous W:Development and validation of flow cytometric measurement for parasitemia in cultures of P. falciparum v itally stained with YOYO-1. Cytometry A2007,71(5):297–307.PubMed click here 48. Myrick A, Munasinghe A, Patankar S, Wirth DF:Mapping of the Plasmodium falciparum multidrug resistance gene 5′-upstream region, and evidence of induction of transcript levels by antimalarial drugs in chloroquine sensitive parasites. Mol Microbiol2003,49(3):671–683.CrossRefPubMed Forskolin 49. Golightly LM, Mbacham W, Daily J, Wirth DF:3′ UTR elements enhance expression of Pgs28, an ookinete protein of Plasmodium gallinaceum.Mol Biochem Parasitol2000,105(1):61–70.CrossRefPubMed Authors’ contributions BB, SM and DAS performed the transfections. BB, CC and SM performed
the growth rate experiments. BB, CC, JCK, and JHA analyzed the insertions data. BB, CC, SM and JHA analyzed the growth rate data. CC, JCK and MJF contributed reagents/materials/analysis tools. BB, CC and JHA drafted the manuscript. BB, MJF and JHA conceived and designed the study. All authors read and approved the final manuscript.”
“Background One of the major sources of human Salmonella infection is meat, including pork and poultry [1, 2] and therefore efficient and rapid monitoring of Salmonella in the meat production chain is necessary. Traditional bacteriological detection of Salmonella in foods and environmental samples is costly, laborious, and time-consuming, requiring 3–7 days to obtain a confirmed result [3]. Thus, rapid and cost-effective detection of Salmonella is of major interest to the food industry and the public.