5 days after the first treatment, ritonavir. Measurement of gastric emptying of rats in each K Provisional set with free access to food and water. The animals were fasted 24 h before the experiment left, but free access to water, to 2 h before the experiment. There was between five and eight rats in each group, the vehicle baicalensis, Hesperadin group ritonavir ritonavir and groups and p. At time 0 h ritonavir was administered orally by gavage. Treatment was twice grass administered intraperitoneally 30 minutes before the time 0 and 120 min. Gastric emptying was measured using the method described above. In short, a. 5 ml of a test meal was incubated with a probe through a tube 3 after oral ritonavir. 30 minutes after a meal, the animals were administered get by CO2 Tet inhalation.
The stomach was rapidly and sorgf validly removed by laparotomy, homogenized in 100 ml of 0th 1 M NaOH for the measurement of gastric emptying. After leaving the stand homogenate for 1 hour at room temperature, the proteins were Executed falls by 0. OSI-930 5 ml of trichloroacetic acid, Centrifuged and separated. The supernatant was mixed with an equal volume 0th Measured 5 M NaOH and the absorbance of the sample at 560 nm with a spectrophotometer. Council offer stomach required standard for the calculation of gastric emptying get Tet were immediately after the test meal was fed. The percentage of gastric emptying was calculated as follows: gastric emptying / ? 100th Thus, the proportion of gastric emptying barrels is to entw best. Statistical Analysis Data were analyzed by SigmaStat third 0 with an analysis of variance.
Significance was assumed at P 0. 05th HPLC results for S. baicalensis extract a chromatogram S. baicalensis extract is shown in Figure 1B. Peaks in the chromatogram scutellarin, baicalein and wogonin were compared by HPLC with an L Considered standard solution of flavonoids. HPLC analysis showed that 1 mg of S. baicalensis extract contained about 9. 3 g ba Cal??ine, one of the most active flavonoids in the extract. The scutellarin flavonoids and wogonin were third in a concentration of 2 and 3 g 3 g ?, Or. Effect of S. baicalensis Ritonavir pica Our previous work has shown that ritonavir at a dose of 20 mg kg ? induced significant contribution kaolin. We best Term that pica significantly induced from 24 to 96 h after oral ritonavir. We also have best Firmed that S.
baicalensis reduced ritonavir-induced pica. In this study, we found that pretreatment with 1 mg ? kg S. baicalensis significantly the intensity t and duration of pica induced by ritonavir. Food intake was not significantly affected. Effect of baicalein ritonavir baicalein-induced pica, an active component of the flavonoids found in S. baicalensis, was tested for its anti-pica baicalensis and their contribution to the action of the extract of S.. Our data indicate that ritonavir-induced pica baicalein significantly reduced in a dose-dependent-Dependent manner. The liquid surface Under the curve from time 0 h kaolin consumption were h for the vehicle group, the group to 120 ritonavir ritonavir group baicalein and baicalein ritonavir group GH 82 15, 355 32 and 237 13 GH gh gh 184 12